Fig 1: High ZIP13 protein level in ovarian cancer is associated with poor outcome. a IHC analysis of ZIP13 expression. b Kaplan-Meier survival analysis of OS in ovarian cancer samples from a tumor tissue microarray (TMA)
Fig 2: ZIP13 promotes ovarian cancer metastasis via the Src/FAK pathway. a. KEGG pathway enrichment analysis for ZIP13-regulated genes. b, c. Quantitative RT-PCR analysis of target genes identified in the RNA-seq. d. Western blot analysis of Src/FAK signaling pathway. e. Correlation analysis of the expression of ZIP13 and target genes in ovarian cancer tissues from TCGA. Spearman’s correlation coefficient and P values were shown for each analysis. *P < 0.05
Fig 3: ZIP13 promotes ovarian cancer cell migration, invasion and adhesion in vitro. a, b. The transwell invasion and migration assays were conducted using the 24-well transwell chambers. The migratory or invasive cells were visualized and counted in at least three random fields under a microscope. c, d. A vertical wound was made through confluent monolayer cells. Then the wound areas were photographed and quantified after 48 h. e, f. Calcein-AM-labeled cells were seeded into 96-well plates pre-coated with either Matrigel or fibronectin (FN), and allowed to adhere at 37 °C for 30 min. The fluorescent signal of the adherent cells was imaged under the microscope and quantified with a fluorescent plate reader. Results indicated were mean values of three independently repeated experiment. *P < 0.05
Fig 4: ZIP13 knockout suppresses peritoneal dissemination of ovarian cancer cells in vivo. a SKOV-3 cell suspensions were injected into peritoneal cavities of BALB/c nude mice (n = 6 mice per group). Representative photos of ascites, tumor formation at the peritoneal cavity and mesenterium were shown. b The ascites was collected and measured. c, d. The disseminated tumors in the abdominal cavities were estimated and quantified. *P < 0.05
Fig 5: ZIP13 knockout inhibits the growth of ovarian cancer cells in vitro. a, c. Cell proliferation assay was performed using CCK-8. At 0, 24, 48, 72 and 96 h, CCK-8 solution was added to each well and OD450 values were measured by a microplate reader. All conditions were tested in six replicates. b, d. SKOV-3 and HO-8910 PM cells were plated in 6-well plates and cultured for 2 weeks. Then the colonies were fixed and stained. The colony number was counted under an optical microscope. Each experiment was independently conducted at least three times. *P < 0.05
Supplier Page from Abcam for Anti-ZIP-13 antibody